Viewers who don't want to see ads can pay an extra $3 a month for an ad-free experience.
Amazon mentioned that ads on Prime Video will be "limited," but didn't specify how long they will be or how many will air per hour.
Ads will no longer be exclusive to live sports like NFL games; they'll also appear in popular shows and movies.
Initially, ads will roll out in the U.S., U.K., Germany, and Canada, with more countries to follow later in the year.
Amazon will offer an ad-free subscription option for $2.99 per month in the United States, with prices for other countries to be revealed.
Prime members will receive advance notification about the ads, allowing them to choose the ad-free option.
The cost of a full Amazon Prime membership is $14.99 per month or $139 per year.
For those only interested in Prime Video, there's a $8.99 per month option.
The changes provide viewers with options: pay less with ads or a bit more for an ad-free streaming experience.